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June 17, 2013
 

Is it possible HIV transmission by micropipette?

Dear Ask The Doctor: Dear Doctor I am 30 years old male from the Philippines.My question is regarding blood extraction via finger prick.The med-tech use a calibrated pipette pump with a replaceable tip to collect blood from my finger instead of a capillary tube.While waiting for me result I had a chat with the med-tech and told me that someone turned reactive and even showed me the test kit that was used.I was not worried at that moment because I still dont know anything about a pipette pump till I got home and searched about the test kit.After reading I very happy knowing the kit was 99.9% according to UNAIDS.What struck me was when I saw the instruction that a pipette pump was only used to get a serum not whole blood from a finger.I searched the internet again and I couldnt find any article regarding the use of pipette pump or micropipette in blood extraction.What I am worried if its possible that the pump had been contaminated with HIV blood because it was used with someone with HIV?I know it has a replaceable tip but what if the blood would reach the shaft where the tip is being placed.And is micropipette are only used in transferring specimens from containers and not collect from a subject patient.Thank you so much

Dear Mark: The following factors are associated with an increased risk of acquiring HIV infection: Unprotected sex, receptive anal intercourse carries a particularly high risk, injection drug use (sharing needles or drug paraphernalia), occupational needle stick or body fluid splash (estimated transmission rate <0.3%), contaminated blood products (before 1985 in the United States). As you see the risk of transmission in the way that you described is extremely low. Every lab has to follow standard  hygienic, sterilization procedures, and yes it is true, is very unusual the micropipettes for draw blood. The HIV virus needs to be inside the white cells and use their internal structures to replicate and survive. No sooner the body fluid containing the HIV virus starts to dry, 90% to 99% of the virus present becomes disabled upon drying. The HIV virus is very fragile when is outside the human body, like on the floor or on an outer surface and is not likely to survive an important amount of time in outside world. The only studies on the survival of HIV outside the body have been done in the laboratory under controlled scientific conditions. These studies have shown that HIV is not affected by extreme cold, but it is destroyed by temperatures of 60 degrees centigrade and above, detergents or disinfectants (bleach).

Last Updated ( Monday, 10 January 2011 )
 
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