When to take a pregnancy test, other reason for missed periods

Patient : My wife, 30, has had regular periods every 28 to 30 days since she was 13. She's never missed periods before. And she's never had any experience with physical intimacy before she met me. We have never had penetrative intercourse yet, though we have had lot of foreplay and oral intimacy. I have also spilled close to or on her vaginal opening while trying to penetrate. We have not been using any contraception - pills for her, condoms for me etc. Usually we cleaned up after I'd spill though we may have missed a few times. Her breasts have enlarged noticeably since we started love-making 2 months back. Now my wife has missed her regular period about 7 days late now. Query: If she is pregnant when would be a good time to take a pregnancy test (how many days after her usual dates of periods) ? Could she have missed her periods because she'd never been sexually intimate with anyone or experienced orgasms ?
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